Topic: CLONING AND EXPRESSION OF THE ISOCITRASE DEHYDROGENASE GENE OF SACCHAROMYCES CEREVISIAE IN ESCHERICHIA COLI.

Order Description

5 ABSTRACT
The abstract should be no longer than 200 words.
No references.
Don’t give explanations for use of methods.
No need to explain how methods work.

Background.
Experimental strategy.
Findings

WRITE THE ABSTRACT LAST!!

1 INTRODUCTION
Points to include in your Introduction.
Why do we clone DNA? What are some applications of DNA cloning?
How do we clone pieces of DNA?

What is this experiment about?
What is the aim of the experiment.?

The abstract and Introduction must be no longer than 2 pages.

2 RESULTS

Analysis of Plasmid DNA by electrophoresis
Why did you generate this data? What are you trying to show?
What did you do to generate this data? (do not give a step by step account of the method)

Present the photograph of your gel electrophoresis (available from LMS).
Make sure it is appropriately labelled and the title is informative (see the section on reports in your lab manual).

What does the data tell us. State your conclusion. Don’t just describe the data!! Based on this data can we go ahead with the experiment? How do you conclude this?

Transformation of E. coli.
Why did you generate this data? What are you trying to show?
What did you do to generate this data? (do not give a step by step account of the method)

Present a table showing your transformation results (the raw data is available on LMS). You only need to present one table. You do not need to present the results of both the 20 µL AND the 100µL samples as they are essentially duplicates of each other (see your manual). For some reactions you can use the data from the 20 µL samples whilst for other reactions you can use the data from the 100 µL samples.

Do not present the raw data for the Table. Process the results and show as transformants/ng plasmid DNA, or as transformants / ml.

What does the data tell us?

Isolation of plasmids from transformants and analysis of structure.
Why did you generate this data? What are you trying to show?
What did you do to generate this data? (do not give a step by step account of the method)

Present the photograph of your gel electrophoresis (available from LMS).
Make sure it is appropriately labelled and the title is informative.

What does the data tell us. State your conclusion. Don’t just describe the data!!
Based on this data was the experiment successful.

3 DISCUSSION.

What do the controls 4-7 tell us?
Why do we get so many more colonies for reaction 4than for reactions 1-3 despite using the same amount of DNA (think topological properties of the DNA)
Compare results for reactions 5 and 6, what do these tell us?
What does reaction 7 tell us?

Spectrum of action of ampicillin. Some bacteria are innately resistant. Its active vs a number of G+ and G- bacteria but not all species are affected. The white colonies may be contaminants. Expand on this point.

Are we likely to recover all fragments equally well? Can we recover the large fragments as easily as the small? How does size affect transformation efficiency?

Improvements, combinational cloning, less complex and involved. Why is it better?

THE DISCUSSION MUST BE NO LONGER THAN 2 PAGES.

4 REFERENCES

Use Endnote Harvard style to format your citations and bibliography.